柳珊瑚Anthogorgia caerulea相关可培养细菌抗污活性筛选

摘要

用4种不同培养基分离纯化柳珊瑚Anthogorgia caerulea中的相关可培养细菌.对获得的90株细菌,先用4株海洋污损指示菌进行活性初筛,获得11株具有抑制海洋污损指示菌生长的细菌后,再用2种抗海洋污损生物幼虫进行活性复筛,复筛得到5株对海洋污损生物幼虫附着有抑制活性的细菌.这5株细菌经16S rRNA基因序列分析,确定其系统发育地位为芽胞杆菌属(Bacillus sp.).%Based on partial morphological analysis, 90 bacterial strains were isolated from the Gorgonian Anthogorgia caerulea obtained from Weizhou island, Guangxi, by means of maine nutrient agar,marine agar 2216,improved LB sea agar and sea water agar. Among them, 11 bacterial strains had antifouling activities against four fouling bacteria, furthermore, 5 bacterial strains effectively inhibited larval settlement of B.neritina and B. amphitrite larvae. Five strains were selected to perform a phylogenetic analysis based on 16S rRNA gene sequences, -and the results showed that these 5 isolates belong to Bacillus genus ( Bacillus sp. ).