高耐受性拜氏梭菌的离子束诱变选育

摘要

Objective]The tolerance to toxic substances of Clostridium beijerinckii was studied in order to optimize bio-butanol industrial production.[Methods]A mutant strain C. beijerinckii GZ-9 was obtained by ion beam implantation and screening.Its inhibitors tolerance and butanol production were compared with that of the original strain C.beijerinckii NCIMB 8052.[Results]The results showed the strain NCIMB 8052 could not grow in the fermentation broth with the phenol concentration of 1 .5 g/L whereas the mutant GZ-9 grew well in fermen-tation medium with 2.4 g/L phenol.When the total soluble phenolic concentration was 1.5 g/L,the experiment yields of the total solvents and butanol production reached to 9.2 g/L and 6·5 g/L.[Conclusion]The mutant strain GZ-9 had strong tolerance to the toxic substances in non-detoxified acid sugar cane bagasse,which was much higher than the original strain NCIMB 8052.%【目的】为了优化生物丁醇生产工艺，研究拜氏梭菌（Clostridium beijerinckii ）对有毒物质的耐受性。【方法】利用常温等离子诱变技术选育获得1株突变株C．beijerinckii GZ-9，并通过摇瓶发酵对比原始菌株C． beijerinckii NCIMB 8052和突变株 GZ-9对有毒物质的耐受性。【结果】NCIMB 8052在酚浓度为1．5 g／L的发酵液中已不能生长，而 GZ-9在酚浓度2．4 g／L的发酵培养基中生长良好；当可溶性总酚浓度为1．5 g／L时，实验总溶剂产量和丁醇产量分别达9．2 g／L和6．5 g／L。【结论】突变株 GZ-9对未脱毒甘蔗渣酸解糖液中毒素物质的耐受性远高于原始菌株 NCIMB 8052。