小月柳珊瑚中生物碱类化学成分及其抗骨肉瘤细胞增殖活性研究

摘要

【目的】研究小月柳珊瑚(Menella kanisa )中生物碱类化学成分及其抗骨肉瘤细胞增殖活性。【方法】采用多种分离方法和波谱分析方法,对小月柳珊瑚中生物碱类化学成分进行分离和结构鉴定。采用 MTT 法测试部分生物碱类化合物抗骨肉瘤细胞 U2 OS 增殖活性。【结果】从小月柳珊瑚中分离出7个化合物,分别鉴定为6-(1ˊ-purine-6ˊ,8ˊ-dionyl)suberosanone (1)、1-(3ˊ-carbonylbutyl)purine-6,8-dione (2)、腺嘌呤脱氧核苷(3)、次黄嘌呤(4)、7-甲基腺嘌呤(5)、胸腺嘧啶脱氧核苷(6)、尿嘧啶脱氧核苷(7)。化合物1和2对骨肉瘤细胞 U2 OS 增殖显示出抑制活性,IC50值分别为13.3μg/mL 和55.0μg/mL。【结论】化合物1~7均首次从小月柳珊瑚中分离得到,其中化合物1和2的抗骨肉瘤细胞活性也为首次报道。%[Objective]The alkaloids from marine gorgonian Menella kanisa and their activities of anti-proliferation on U2 OS cancer cell were investigated.[Methods]The alkaloids were isolated and purified by column chromatography on silica gel,and their structures were identified by spectral analyses and compared with literatures. The cytotoxicity of compounds 1 ~2 toward hu﹣man osteosarcoma cell U2 OS cancer cell line was evaluated by MTT methods.[Results]Seven al﹣kaloids were obtained from M.kanisa and char﹣acterized as 6-(1ˊ-purine-6ˊ,8ˊ-dionyl)suberos﹣anone (1),1-(3ˊ-carbonylbutyl)purine-6,8-dione (2),adenine deoxyribonucleoside (3),hypoxanthine (4),7-methyladenine (5),thymine de﹣oxyribonucleoside (6)and uracil deoxyriboside (7).Compounds 1 and 2 showed moderate cyto﹣toxicity against osteosarcoma U2 OS cell line with IC50 of 13.3 and 55.0 μg/mL,respectively.[Conclusion]Compounds 1 ~ 7 were isolated from gorgonian M.kanisa for the first time.The antitumor activity of Compounds 1 and 2 against U2 OS in vitro was also reported for the first time.