榕树须醇提取物对脑血栓形成和凝血功能的影响

摘要

Objective]This study observed the effects of different extract substances from the aerial roots of Ficus microcarpa on coagulation,as well as the effect on cerebral thrombosis.[Methods]The mice model of cerebral thrombosis was induced by collagen protein and the rat thrombotic model was induced by electric stimulation,which were used to examine the effects of extract substances on the protective rate of mice cerebral thrombosis and rat thrombotic for-mation,respectively.The mice model of blood stasis was established by combined application of epinephrine with ice bath,which was used to observe the influences of the compounds on thrombin time and fibrinogen time.[Results]Different extract substances from 95% ASARFM (alcohol-soluble substance from the aerial roots of Ficus microcarpa )or 80% ASARFM could increase the recovery rate of cerebral thrombosis in mice (20%~60%).Both acetidine-soluble extract substance and n-butanol-soluble substance had better protective effect on the model of cerebral thrombosis by electric stimulation (P<0·01 and P <0·001),and could delay thrombin time of the mice with cold and stasis (P <0.05 and P <0.001 );N-butanol-soluble substance could delay fibrinogen time of the mice with cold and stasis (P <0.05).[Conclusion]N-butanol-soluble substance extracted from 80% ASARFM had better protective effect on the model of cerebral thrombosis and could improve the coagulation of mice.%【目的】探讨榕树须不同提取物的抗脑血栓作用,并评价其对凝血功能的影响。【方法】采用小鼠脑偏瘫、电刺激致大鼠血栓模型观察提取物对小鼠脑血栓的保护率、大鼠血栓形成时间的影响；采用肾上腺素加冰浴致小鼠血瘀模型观察其对凝血酶时间及纤维蛋白原时间的影响。【结果】榕树须95%、80%醇提物的不同提取部位能提高小鼠脑偏瘫恢复率(20%~60%)；乙酸乙酯及正丁醇部位对电刺激引起的脑血栓有较好保护作用(P <0.01,P<0.001)；乙酸乙酯部位及正丁醇部位均能延长寒凝血瘀小鼠的凝血酶时间(P <0.05,P <0.001)；正丁醇部位能延长寒凝血瘀小鼠的纤维蛋白原时间(P<0.05)。【结论】榕树须80%醇提物的正丁醇部位具有较好的抗脑血栓及改善血瘀小鼠凝血功能的作用。