Objective To investigate the expressions of human β-defensin-2( hBD-2 )in oral epithelium in patients with oral lichen planus( OLP ) , and explain the pathomechanism of hBD-2 in OLP and inflammatory response of epithelium. Methods Oral mucous tissue samples from 25 OLP patients and 7 health controls were included in this study. ImmunohistochemistrY( IHC )and reverse transcription-polymerase( RT-PCR )were used to evaluate the expression of hBD-2 separately. Results Positive stains of hBD-2 were observed in all the samples by IHC method. The reaction intensity in OLP group was strong,while in health controls it showed weak signal,which showed statistical difference ( P ＜0. 05 ). By RT-PCR,hBD-2 were expressed in cDNA electrophoresis strips both in health controls and patients. The expressions levels of hBD-2 mRNA in OLP patients were significantly higher than those of health controls( P ＜0. 05 ).Conclusion The small amount of expressions of hBD-2 on normal oral mucosa,which suggests that it play a role in maintaining the health of oral mucosa as a part of innate immunity. The up-regulating expression of hBD-2 under inflammation station in OLP may participate in the immunity course of OLP.%目的 探讨口腔扁平苔藓(OLP)患者病损中的人类β-防御素2(hBD-2)的表达,以分析hBD-2在OLP发病机制与上皮抗感染免疫中的作用.方法 采用免疫组织化学技术(IHC)和反转录聚合酶链式技术(RT-PCR),检测25例OLP病损组织、7例对照组织中hBD-2多肽的表达,并进行分析.结果 hBD-2在所有样本中均出现了阳性胞浆染色,健康口腔黏膜组中hBD-2蛋白为弱阳性染色,与呈强阳性染色的OLP组差异有统计学意义(P＜0.05);RT-PCR检测结果显示,所有样本均出现了hBD-2的cDNA电泳条带,在健康口腔黏膜组中呈微弱表达,在OLP组织中表达明显增强,差异有统计学意义(P＜0.05).结论 健康口腔黏膜组织也有少量hBD-2表达,提示其作为机体先天性免疫的组成部分,在维持口腔黏膜健康上具有一定作用.在OLP的病理过程中hBD-2可能呈炎性诱导上调表达而参与了OLP的免疫发病过程.
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