小鼠鼻气道阻力检测方法的建立

摘要

Objective To introduce a simple and available method for measurement of nasal airway resistance ( RNA) and nasal lavage in mice .Methods Sixteen female mice were randomly divided into 2 groups:allergic rhinitis (AR) group and control group ,with 8 mice in each group.AR mice were immunized by intraperitoneal injection of ovalbumin plus suspension of aluminum hydroxide and normal saline as well as challenged with intranasal ovalbumin plus the suspension above .Control mice were sensitized by systemic intraperitoneal injection of normal saline and then challenged with intranasal normal saline .All mice were anesthetized after challenge .A cannula (18G) was inserted into the trachea .A blunt needle connected with a T tube was inserted into nasopharynx .One end of the T tube was connected to a syringe pump which provided consistent air flow to the nasal passages .Another end of the T tube was connected to a pressure transducer for measurement of pressure difference across nasal passage .Pressure difference was divided by air flow to give values of RNA .Histamine was nebulized into nasal cavity to measure nasal response .A polyethylene catheter connected to a syringe was gently inserted into one nostril and the fluid was simultaneously collected from the contralateral nostril.Nasal lavage cytology and nasal histology were measured .Results The basic and postchallenge RNA values as well as nasal response values for AR group were higher than those of control group (allP<0. 01) .Eosinophils and neutrophils in nasal lavage fluid increased significantly in the AR group compared with the controls (all P<0 .01) .Obvious infiltration of eosinophils and mild edema were observed in the nasal mucosa in the AR group , but weren't observed in the control group .Conclusion We establish a relatively simple and available method for measuring nasal airway resistance and nasal lavage in mice .%目的：建立简单易行的小鼠鼻气道阻力检测方法及鼻灌洗方法。方法雌性小鼠16只，随机分为两组，每组8只，变应性鼻炎组（ AR组）采用卵清蛋白＋氢氧化铝生理盐水混悬液腹腔注射致敏及滴鼻激发致敏，建立变应性鼻炎小鼠模型，对照组用生理盐水腹腔注射致敏和滴鼻激发。致敏后小鼠麻醉分离气管，近喉部切开气管插入18 G插管至鼻咽部，插管连接“T”形管，再分别连接实验精密注射泵和压力传感器，应用灌注/吸引模型模拟呼气/吸气，检测鼻气道的压力，计算鼻阻力。对小鼠鼻腔进行组胺雾化激发，检测鼻气道反应性。采用一侧鼻腔插管灌洗，对侧鼻腔收集灌洗液的方法，对鼻腔灌洗液进行中性粒细胞、嗜酸性粒细胞计数；处死小鼠后取鼻黏膜组织进行病理组织学观察。结果对照组小鼠的基础鼻气道阻力、反应性及鼻激发后的鼻气道阻力、反应性均低于AR组（P均＜0．01）；AR组鼻腔灌洗液中性粒细胞、嗜酸性粒细胞、细胞总数均显著高于对照组（P均＜0．01）；AR组的鼻黏膜有明显的嗜酸性粒细胞浸润，血管轻度充血水肿。对照组小鼠鼻黏膜无明显异常。结论成功建立了简单易行的小鼠鼻气道阻力检测方法及鼻灌洗方法。