精氨酸加压素对大鼠心肌成纤维细胞增殖及其胶原合成的影响

摘要

Objective To explore the effect of arginine vasopressin( AVP) on the proliferation and collagen synthesis of cardiac fibroblasts( CFs) .Methods SD rats were given AVP by intravenous injection.Masson staining was performed to assess myocardial fibrosis, and Western Blot was used to detect the expression of transforming growth factor( TGF)-β1.CFs were isolated from neonatal SD rats by collagenase digestion.MTT assay was used to determine the proliferation of CFs,and 3 H-proline incorporation to determine the collagen synthesis of CFs.Results SD rats presented myocardial fibrosis when treated with AVP continuously with the increasing expression of TGF-β1.Both MTT optical density and 3 H-proline incorporation rate of CFs increased gradually with the increasing concentration of AVP. And they were significantly higher than the controls when the concentration of AVP was 10 -6 mol/L(P<0.05).MTT optical density and 3 H-proline incorporation rate of CFs increased gradually with the increasing treatment time based on the intervention of 10 -7 mol/L AVP. And there was significant difference between 36-hour culture group and 6-hour culture group(P<0.05).Conclusion Intervention with high concentration of AVP for a long time might promote the proliferation and collagen synthesis of CFs by improving the expression of myocardial TGF-β1 in rats,thus resulting in myocardial fibrosis.%目的 探讨精氨酸加压素( AVP)对心肌成纤维细胞( CFs)增殖及其胶原合成的影响. 方法 SD大鼠静脉注射AVP,马松染色检测纤维化程度,Western Blot检测转化生长因子-β1(TGF-β1)的表达. 采用胶原酶消化法分离培养SD仔鼠的CFs,MTT法测定CFs细胞增殖数目,3 H-脯氨酸掺入法测定CFs胶原合成功能. 结果 AVP持续作用会促进大鼠心肌TGF-β1表达增加,从而促进心肌纤维化发生. 随着AVP浓度的升高,CFs的MTT吸光度值( OD)及3 H-脯氨酸掺入率均逐渐升高. 当AVP浓度为10 -6 mol/L时,MTT OD值及3H-脯氨酸掺入率均明显高于对照组(P<0.05). 在10 -7 mol/L AVP干预下,随着培养时间的延长,CFs的MTT OD值及3 H-脯氨酸掺入率均逐渐升高. 在培养36 h时与培养6 h组比较,差异有统计学意义(P<0.05). 结论 高浓度AVP长时间作用可以通过促进大鼠心肌TGF-β1表达,促进CFs增殖及其胶原合成,从而导致心肌纤维化发生.