绞股蓝总皂苷对人肾上腺皮质癌 SW－13细胞增殖及凋亡蛋白 Caspase－8表达的影响

摘要

目的：探讨绞股蓝总皂苷（Gyp）体外干预对人肾上腺皮质癌（ACC）SW－13细胞增殖及其含半胱氨酸的天冬氨酸蛋白水解酶－8（Caspase－8）表达的影响。方法采用 MTT 法检测不同浓度的 Gyp 对 SW－13细胞增殖的影响；普通显微镜下观察细胞形态学改变；实时荧光定量 PCR 检测 Gyp 干预后 Caspase－8表达的变化。结果Gyp 对 SW－13细胞增殖产生明显地抑制作用，且呈时间和浓度依赖性；光镜下观察，Gyp 干预后 SW－13细胞凋亡特征更明显；实时荧光定量 PCR 结果显示，Gyp 干预后 SW－13细胞 Caspase－8 mRNA 表达明显上调（P ＜0．01）。结论Gyp 可显著抑制人 ACC SW－13细胞的增殖，其作用机制可能为通过上调 Caspase－8 mRNA 的表达来实现。%Objective To investigate the effect of gypenosides(Gyp) on the proliferation and Caspase-8 expression of SW-13 cells of human adrenocortical carcinoma in vitro.Methods MTT assay was adopted to detect the effects of different concentrations of Gyp on the proliferation of SW-13 cells,ordinary optical microscope to observe the morphological changes,and real-time quantitative PCR to deter-mine the changes in Caspase-8 expression after the interference of Gyp.Results The growth of SW-13 cells was inhibited obviously by Gyp in a dose-and time-dependent manner.The observation on SW-13 cells by light microscope revealed that apoptosis features of the cells were more significant.The result of real-time quantitative PCR showed that the mRNA expression of Caspase-8 of SW-13 cells up-regulated significantly after the interference of Gyp(P <0.01).Conclusion Gyp can significantly inhibit the proliferation of human adrenocortical carcinoma SW-13 cells,and this might be achieved by up-regulating the mRNA expression of Caspase-8.