Objective To investigate the relationship of gene polymorphism of interleukin-13 ( IL-13 ) with incidence of asthma , serum IgE and IL-13 levels in children with Zhuang nationality .Methods A total 112 healthy children with Zhuang nationality ( control group) and 80 asthma children with Zhuang nationality(asthma group) were enrolled.Polymorphisms of IL-13 gene -1112C/T and +2044A/G loci,serum IgE and IL-13 levels were detected.Results (1) CC genotype and C allele of IL-13 -1112C/T locus were dominant in the asthma and control groups,but no significant difference in the distribution of genotype frequency or of allele frequency was found between the two groups (P>0.05).There was significant difference in the distribution of genotype frequency and allele frequency of IL-13 +2044A/G locus between the two groups(P<0.05),AG genotype and GG genotype were the common genotypes in the asthma group and control group respectively,and the A allele frequency in the asthma group was significantly higher than that in the control group(P<0.05).(2)In the asthma group and control group,there were no significant differences in the serum IgE or IL-13 level among different genotypes of IL-13 -1112C/T or +2044A/G locus (P>0.05).In the three genotypes of IL-13 -1112C/T locus,the serum IgE levels of the asthma group were higher than those of the control group(P<0.05),but there were no significant differences in the serum IL-13 levels in the three genotypes of IL-13 -1112C/T locus between the two groups(P>0.05).In the three genotypes of IL-13 +2044A/G locus,the serum IgE and IL-13 levels of the asthma group were higher than those of the control group(P<0.05).Conclusion Polymorphism of IL-13 +2044A/G locus is associated with the incidence of asthma,may be related to the elevation of IL-13 and IgE levels,and is a susceptible gene of asthma in the children with Zhuang nationality .However,gene polymorphism of IL-13 -1112C/T locus is not associated with the incidence of asthma in children with Zhuang nationality .%目的 探讨白细胞介素-13(IL-13)基因多态性与壮族儿童哮喘发病及血清IgE、IL-13水平的关系.方法 选择112名健康壮族儿童(对照组)和80例壮族哮喘患儿(哮喘组),检测其IL-13基因-1112 C/T和+2044 A/G位点多态性以及血清IgE、IL-13水平.结果 (1)哮喘组和对照组IL-13-1112C/T位点均以CC型为主,等位基因以C为主,两组间基因型、等位基因频率分布比较,差异均无统计学意义(P>0.05);两组IL-13+2044A/G位点基因型、等位基因频率分布比较,差异有统计学意义(P<0.05),其中哮喘组以AG型最常见,对照组以GG型最多见,哮喘组A等位基因频率高于对照组(P<0.05).(2)哮喘组、对照组组内IL-13-1112C/T、+2044A/G位点不同基因型之间血清IgE、IL-13水平比较差异均无统计学意义(P>0.05),哮喘组IL-13-1112C/T位点3种基因型患者的血清IgE水平均高于对照组(P<0.05),而两组间IL-13-1112C/T位点3种基因型患者的IL-13水平比较差异无统计学意义(P>0.05);哮喘组IL-13+2044 A/G位点不同基因型患者的血清IgE水平、IL-13水平均高于对照组(P<0.05).结论 IL-13+2044A/G位点多态性与壮族儿童哮喘发生有关,可能与血清IL-13、IgE水平升高有关,是广西壮族儿童哮喘哮喘发病的易感基因;但IL-13-1112C/T位点基因多态性与壮族儿童哮喘的发病无关联.
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