骨髓增生异常综合征患者骨髓微小RNA-340-3p的表达情况及生物信息学分析

摘要

目的 探讨微小RNA(miR)-340-3p在骨髓增生异常综合征(MDS)患者骨髓中的表达情况,并应用生物信息学技术分析其靶基因及功能.方法 利用基因表达谱芯片结合分层聚类在9例MDS患者和6例健康正常人骨髓中筛选出差异表达的miR-340-3p,并利用miRBase和Miranda软件预测其靶基因,并对预测的靶基因进行功能富集和信号通路分析.结果 与健康正常人比较,miR-340-3p在MDS患者骨髓中表达下调(P<0.05).获得共同76个潜在靶基因,包括BANP、KIF2C、DIDO1、GRM1等.基因本体(GO)分析显示靶基因主要富集于细胞生物学、分子功能、细胞组分,信号通路分析显示其主要富集于FoxO信号通路、NOD样受体信号通路、造血细胞系、Gap连接等信号转导通路等.结论 miR-340-3p在MDS患者骨髓中表达下调,其可能通过靶向负向调控下游的靶基因而参与MDS的发生发展.%Objective To explore the expression of microRNA(miR )-340-3p in bone marrow of patients with myelodysplastic syndrome (MDS),and to analyze its target genes and function by bioinformatics analysis .Methods miR3-40 -3p differentially expressed in the bone marrow of 9 patients with MDS and 6 healthy individuals was screened using gene expression profile chip and hierarchical clustering .Then its target genes were predicted using miRBase and Miranda softwares .Gene Ontology ( GO) analysis and signal pathway analysis were performed in the predicted target genes .Results Compared to the healthy normal individuals ,the expression of miR-340-3p in bone marrow of patients with MDS was down-regulated(P<0.05).A total of 76 potential target genes were obtained ,including BANP,KIF2C,DIDO1, GRM1 and ect..GO analysis showed that the target genes were enriched in cellular biological process ,molecular function and cellular component. And signal pathway analysis showed that these target genes were enriched in FoxO signaling pathway ,NOD-like receptor signaling pathway , hematopoietic cell line ,Gap junction and ect..C onclusion The expression of miR-340-3p decreases in the bone marrow of patients with MDS,and probably participates in the occurrence and development of MDS by negatively targeting the downstream target genes .