益气养阴方对 Graves 病合并白细胞减少小鼠 IL-13表达影响的实验研究

摘要

Objective To observe the effect of Yiqiyangyin decoction on the peripheral white blood cell count and IL -13 expression of GD Mice To discuss the mechanism of yiqiyangyin decoction to merger leukopenia .Methods To establish the model of GD mice by Gene gun . After the therapy of Yiqiyangyin decoction , we observed the peripheral white blood cell count and IL-13 expression alteration of GD Mice . Results After the treatment in 30 days, the peripheral white blood cell count in high-dose group of Yiqiyangyin decoction was significantly higher than low-dose group and middle-dose group ( P <0.01 ) .High-dose group and middle-dose group of Yiqiyangyin decoction was significantly superior to low-dose group in reducing thyroid hormones ,.The tapazole group decreased obviously , which was significant difference with normal group in IL-13 expression(P<0.01).There was no significant difference in IL-13mRNA expression between chinese medicine treatment group and normal group (P >0.05).After the treatment of Yiqiyangyin decoction , the IL-13mRNA expression down regulated,which mean High-dose group express the lowest , with gradually increasing .Conclusions Yiqiyangyin decoction has a better therapeutic effect to Graves disease combining with leukopenia .Its mechanism may be related with better regulating IL-13 expression . Yiqiyangyin decoction can promote the maturation , activation, proliferation, and immunodulatory of immune cell .%目的：观察Graves病（ GD）小鼠外周血白细胞计数和小鼠细胞因子IL-13表达及益气养阴方对其的影响。方法基因枪造模建立GD小鼠模型，应用中药益气养阴方治疗后，观察小鼠外周血白细胞计数和小鼠细胞因子IL-13表达的变化。结果治疗30天后，在外周血白细胞计数方面，中药益气养阴方高剂量组明显高于中药益气养阴方低剂量组、中剂量组（ P ＜0.01）；在降低甲状腺激素水平方面，中药益气养阴方高、中剂量组明显优于中药益气养阴方低剂量组；在小鼠细胞因子IL-13表达方面，西药他巴唑组小鼠细胞因子IL-13表达显升高，与正常对照组和模型组比较有显著差异性（ P ＜0.01）。中药治疗组的IL-13mRNA表达与正对照组比较没有显著性差异，P＞0.05。经中药益气养阴方治疗，小鼠IL-13mRNA的表达下调，即中药高剂量组表达最低，依次增高。结论益气养阴方对Graves病合并白细胞减少有较好的治疗作用，其机理可能在于其能较好地调节Graves病小鼠细胞因子IL-13水平，有促进免疫细胞成熟、活化、增殖和免疫调节的作用。