To study the protective effect of Yubao bio-organic selenium honey on alcohol-induced acute hepatic injury in mice, ICR mice were random divided into seven groups with 10 mice in each group: solvent control, solvent model, honey control, honey model and low, middle, high dosage of Yubao bio-organic selenium honey. The three dosage groups were given selenium honey orally at the dose of 1 667, 3 333, 6 667 mg/(kg/d) daily. The solvent control and solvent model groups were given double evasporated water. The honey control and honey model groups were given honey orally at the dose of 20 mL/kg(bwt) Thirty days later, the model and the three dosage groups were given 50% alcohol at the dose of 10 mL/kg(bwt), the control groups were given double evaporated water. The mice were executed respectively after fasting 12 h. MDA, GSH and TG in liver tissue were measured. The livers were observed by pathologically with frost slice techniques, The result showed that Compared with honey model group, GSH content was increased significantly in low and middle dosage groups(P < 0. 01) , TG content was decreased significantly in all groups (P <0. 01). The fatty degeneration grade in every group was all less than the model groups (P <0. 05). It concludes Yubao Bio-organic selenium honey has the protective effect on liver injuries induced by alcohol in mice.%为研究菀葆牌硒蜜康对小鼠酒精性肝损伤的保护作用,将动物随机分成溶剂对照组、溶剂模型组、蜂蜜对照组、蜂蜜模型组及低、中、高3个样品剂量组,共7组,每组10只,3个样品剂量组每天给予硒蜜康1 667、3 333、6 667 mg/(kg·d),连续给予小鼠灌胃30 d后,模型对照组和样品剂量组最后一次给予受试物后20 min,按体质量计一次灌胃给予50%乙醇10 mL/kg,对照组给双蒸水,禁食12 h 后处死,取动物肝脏,测定肝匀浆液MDA、GSH、TG含量;肝脏冰冻切片,进行病理组织学检查.结果表明,与蜂蜜模型组比较,中、低剂量组的GSH含量升高,各剂量组的TG含量降低,各剂量组冰冻病理学组织切片观察到脂肪变性评分低于模型对照组,差异均有显著性.可见菀葆牌硒蜜康对酒精性肝损伤具有辅助保护作用.
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