目的：观察过氧化物酶体增殖物激活受体-γ（ PPAR-γ）的配体15-脱氧-△12，14-前列腺素J2（15d-PGJ2）对兔耳增生性瘢痕的影响，探讨15d-PGJ2防治增生性瘢痕的机制和可行性。方法选取新西兰大白兔9只，在兔耳腹侧面制作1 cm ×1 cm全层皮肤缺损创面，每耳4个，共计72个，建立兔耳增生性瘢痕动物模型，随机分为实验组和对照组，分别用15d-PGJ2及生理盐水行瘢痕内注射，1次/d，连续注射7 d。停止注射后第3、6、17天取材。应用免疫组织化学方法检测CD34、转化生长因子-β1（ TGF-β1）的表达。结果兔耳创面愈合后均出现不同程度类似人增生性瘢痕组织块，与对照组比较，实验组瘢痕逐渐变平变软，颜色变浅；在各时间点实验组CD34和TGF-β1的表达均较低，与对照组比较差异有统计学意义（ P＜0.05）。结论 PPAR-γ的配体15d-PGJ2可减少瘢痕内CD34、TGF-β1的含量，引起瘢痕萎缩，有一定的防治瘢痕增生的作用。%Objective To investigate the effect of 15-deoxy-△12,14 -prostagliandxin J2 (15d-PGJ2) on the expression of CD34 and TGF-β1 on hypertrophic scar in rabbit ears , and the possibility of 15d-PGJ2 in treatment of hy-pertrophic scar .Methods Ear hypertrophic scar model was established in 9 New Zealand white rabbits .Four wounds with total skin loses (1 cm ×1 cm) on the ventral side on each ear were constructed in all 9 rabbits.The wounds were randomly divided into the 15d-PGJ2 treatment group and control group , in which 10 μL 15d-PGJ2 and normal saline, respectively, were injected into the ear scar once a day for 7 days.On the 3rd, 6th and 17th day after the injection, 12 scars of each group were harvested .The expression of CD34 and TGF -β1 was detected by immunohistochemical method . Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar were observed in the both two groups.Compared with control group , smaller, softer, flatter and color lighter scars were observed in treatment group .The expression of CD34 and TGF-β1 in the 15d-PGJ2 group was significantly down -regulated as compared with that in control group at different time points (P<0.05).Conclusion The 15d-PGJ2, ligand of PPAR-γ, can reduce the ex-pression of CD34 and TGF-β1 of hypertrophic scar in the rabbit ears and plays an important role in the prevention and treatment of hypertrophic scar .
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