四个产地石菖蒲药材挥发油中α-细辛醚含量对比研究

摘要

Objective： To determine a-asarone in Rhizoma Acorus tatarinowii Schott from four origins. Methods： GC condition consists of 100 % DPS column（ 18 m×0.32 mm×0.25 μm）, the temperatures of injector and detector with 200℃ and 260 ℃respectively, sample size with 1 μL, flow in 1.51 m/min, split ratio of 40：1, acetic ether as solvent, highly pure nitrogen as carrier gas and n-octadecane as internal standard. Results： Assay of a-asarone showed the Acorus tatarinowii from HuNan contains relative high and stable levels of a-asarone. The linear equation of α-asarone： y=0.6297x＋0.0053, r2=0.9999（n=6） was obtained in the range of 0.0227-0.4432 mg/mL. The recovery of α-asarone was 94.2 %. Conclusion： The method was accurate, rapid and sensitive.%目的：测定四个产地石菖蒲中＊细辛醚的含量进行对比。方法：色谱条件：100％=：甲基聚硅氧烷分析柱（18m×0．32mm，0．25μm）；升温程序：起始温度60℃，保持5min，6℃／min升至200℃：进样口温度：200℃：检测器温度：260℃：进样量：1儿；流速：1．51m／min；分流比：40：1：溶剂：乙酸乙酯；载气：氮气；内标物：正十八烷。结果：湖南产石菖蒲中α-细辛醚的含量相对高。α-细辛醚回归方程：Y=0．6297X＋0．0053，r2=0．9999：线性范围：22-443μg．mL^-1；α-细辛醚的回收率为94．2％。结论：方法快速准确，稳定，样品处理简便，易。