盐分胁迫对5种园林灌木生理生化指标的影响

摘要

The impact of salt solution at different concentrations on five garden shrubs（Grandiflora,Maculata,Casin,Rosmarinus and Makino was studied）.The result indicated that the chlorophyll and carotenoids contents were reduced along with the salt concentration.And the decline range of Grandiflora and Makino was small.The chlorophyll content variation of Makino was the minimum.The five garden shrubs contents of SOD,CAT,POD and soluble protein were negatively correlated with the cell membrane permeability.The enzyme active of Makino and Rosmarinus was higher.The soluble protein content was not important in salt resistance.The contents of proline,soluble sugar,MAD activity were negatively correlated with cell membrane permeability.Makino,Rosmarinus and Grandiflora showed an increasing trend.Their superoxide radical changes were small,membrane damage showed a lesser degree.Maculata and Casin showed a poor salt tolerance.%通过对不同浓度盐胁迫下5种园林灌木大花六道木、滨柃、花叶胡颓子、伞房决明和迷迭香生理生化指标的测定,结果表明,随着盐分的升高,5种园林灌木的叶绿素和类胡萝卜素含量不断下降,大花六道木和滨柃的下降幅度较小,分别为28.8%和17.8%,滨柃的叶绿素含量的变化幅度最小,为21.2%和6.5%。5种园林灌木SOD、CAT、POD酶活性和可溶性蛋白含量与细胞膜透性呈显著或极显著负相关,滨柃具有较强的酶活性,上述指标最高时可分别达258.0%,58.6%和184.0%。可溶性蛋白对植物的耐盐程度作用不大。5种园林灌木的游离Pro、可溶性糖、超氧阴离子自由基含量和MDA均与细胞膜透性呈极显著和显著相关。在重度盐分胁迫下,滨柃的脯氨酸含累量最大,为对照的6倍,可溶性糖的含累量仅次于大花六道木。超氧阴离子自由基含量和MDA在体内的变化幅度最小,达90%和18.71%,其次是大花六道木和迷迭香。因此,细胞膜受伤害程度最小的是滨柃、其次是大花六道木、迷迭香。花叶胡枝子和伞房决明的耐盐能力较弱,不适应盐分较高地区种植。此研究为园林灌木的地区选择提供了一定的理论依据。