肺胀阳虚水泛证动物模型的建立方法

摘要

目的 探讨肺胀阳虚水泛证动物模型的造模方法.方法 大鼠随机分为空白对照组;氢化可的松对照组;阿霉素对照组;阿霉素低剂量组;阿霉素高剂量组.序贯应用香烟反复熏吸、气管内注入脂多糖、野百合碱腹腔注射、氢化可的松肌肉注射及阿霉素腹腔注射对大鼠进行造模,然后观察大鼠的精神、形态、活动等,测量尿量,采用心脏超声心动图分阶段检查心脏结构及功能,最后切开胸腹腔探查胸腹脏器情况.结果 应用香烟反复熏吸、气管内注入脂多糖、野百合碱腹腔注射后可引起大鼠疲倦、消瘦、咳嗽、喘促等慢性支气管炎的表现,氢化可的松肌肉注射后可以出现大鼠畏寒、弓背、缩身、体毛枯疏蓬松等肺阳虚表现,阿霉素腹腔注射后可以出现大鼠心肌肥厚、心脏扩大、射血分数下降,严重者形成心包积液、胸腔积液、腹腔积液等水泛表现.结论 序贯应用香烟反复熏吸、气管内注入脂多糖、野百合碱腹腔注射、氢化可的松肌肉注射及阿霉素腹腔注射对大鼠进行造模,可以形成肺胀阳虚水泛证动物模型.%Objective To discuss the establishment method of animal model with water over-flowing syndrome due to Lung-yang asthenia. Methods The eperimental rats were divided into five groups, blank controller, hydrocortisone group, doxorubicin controller, low-dose doxorubicin group and high-dose doxorubicin group. All rats were passively smoked repeatedly, and were injected in sequence with lipopolysaccharide intratracheally, monocrotaline intraperitoneally, hydrocortisone intramuscularly, doxorubicin intraperitoneally. Then the activities, shape and spirit of rats were observed, the volume of urine were, determined, the cardiac structures and function were checked by heart ultrasound, and finally the rats were dissected to examinate the internal organs's conditions in the thoracic cavity and abdominal cavity. Results Repeatedly passively smoking, lipopolysaccharide intratracheally injection and monocrotaline intraperitoneally injection lead to fatigue, weight loss, cough, breathlessness, which were the clinical manifestation of chronic bronchitis. Hydrocortisone intramuscularly injection leads to chills, arched back, shrinking body, sparse fur and other lung Yang deficiency demonstration. Doxorubicin intraperitoneally injection leads to myocardial hypertrophy, cardiac dilatation, low ejection fraction and even pericardial effusion, pleural effusion and pyoperitoneum, which are the clinical manifestations of water over-flowing syndrome. After that the rats injected by would have so much as. thatConclusions We can estabishingThe animal model of over-flowing syndrome due to Lung-yang asthenia can be established by using the following methods in sequence, firstly passively smoked repeatedly, secondly injected by lipopolysaccharide intratracheally and monocrotaline intraperitoneally, then hydrocortisone intramuscularly, endly doxorubicin intraperitoneally.