清热解毒方对2型糖尿病模型大鼠血清炎症因子TNF-α、IL-1、IL-6的干预研究

摘要

目的 观察清热解毒方对2型糖尿病大鼠血清肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-6(IL-6)水平的影响.方法 采用小剂量链脲佐菌素(STZ)加高脂饮食饲养的方法建立2型糖尿病(DM)肥胖大鼠模型,随机分为正常对照组,模型组,中药高剂量组,中药低剂量组,二甲双胍组.给药4周后观察大鼠血清TNF-α、IL-1、IL-6水平的变化.结果 与模型组相比,中药组血糖、血清TNF-α、IL-1、IL-6水平明显下降(P<0.05),中药高低剂量组之间无明显差异,中药组与二甲双胍组相比有显著差异(P<0.05).结论 清热解毒方可明显降低2型糖尿病模型大鼠血清中的炎症因子水平.%Objective To study the influence of Qingre Jiedu Decoction on type 2 diabetic rat serum tumor necrosis factor-a and interleukin-6 levels. Methods To establish type 2 diabetic obese rat model by injecting with low dose of STZ and high-fat diet feeding. Then the rats were randomly divided into model group, high dose of Chinese medicine, low dose of Chinese medicine and metformin group. After 4 weeks drug delivery, the levels of tumor necrosis factor-a and interleukin-6 in the rat serum were measured. Results Compared with the model group, the serum tumor necrosis factor-a and interleukin-6 levels of Chinese medicines groups were decreased significantly. There is no significantly difference between the two groups of traditional Chinese medicine group. There is a significant difference between the metformin group and the TCM group. Conclusion Qingrejiedu Decoction can significantly reduce the levels of tumor necrosis factor-ct and interleukin-6 in the serum of T2DM rat.