间接竞争酶联免疫分析法测定葛根中葛根素的含量

摘要

Objective To detect the content of puerarin in puerariae lobatae radix by ic-ELISA. Methods Anti-Puerarin monoclonal antibodies were used to establish ic-ELISA method for puerarin by in-vestigating the linear relationship, the precision and the recovery. Results The linear range of this method was 15. 6 ng/mL~500 ng/mL, the coefficients of variation for the intra-assay and the inter-assay are both less than 3. 5% and the average recovery was 101. 8%. The detection result of ic-ELISA method was consistent with that of HPLC test. Conclusions The ic-ELISA method can be applied to the quality control of puerariae lobatae radix and the content determination of puerarin in Chinese herbal formula.%目的：利用间接竞争酶联免疫分析法( indirect competitive enzyme linked immunosorbent assay, ic-ELISA)测定葛根中葛根素的含量。方法利用抗葛根素单克隆抗体,通过线性关系、精密度、回收率的考察,建立葛根素的ic-ELISA分析方法。结果该方法的线性范围为15.6~500 ng/mL,孔间差和板间差均不大于3.5%,平均回收率为101.8%,并且该法检测结果与高效液相色谱法检测结果一致。结论本实验为葛根质量控制以及含葛根药材的中药复方中葛根素的含量测定提供了一种新技术方法。