连夏配方颗粒对心肌梗死后大鼠心梗周围区神经生长因子mRNA、酪氨酸激酶受体A mRNA表达的影响

摘要

目的观察连夏配方颗粒对心肌梗死后大鼠心梗周围区心肌组织神经生长因子( nerve growth factor, NGF) mRNA、酪氨酸激酶受体A( tyrosine kinase receptor A,TrKA) mRNA表达的影响。方法将90只清洁级健康7周龄Wistar雄性大鼠随机分为假手术组、心梗模型组、美托洛尔组、连夏配方颗粒高、中、低剂量(189．00、94．50、47．25 mg/kg)组。各组均结扎左冠状动脉前降支以复制心肌梗死大鼠模型,假手术组只穿线,不结扎。假手术与心梗模型组大鼠予生理盐水,余各组予相应实验药物；连续灌胃给药30天,采用Real-time RT-PCR方法检测心梗周围区NGF mRNA、Tr-KA mRNA表达水平。结果与假手术组比较,心梗模型组大鼠心梗周围区心肌组织NGF mRNA、TrKA mRNA表达均明显增高(P<0．01)；与模型组比较,连夏配方颗粒高、中、低剂量组大鼠心梗周围区NGF mRNA表达均明显降低(P<0．01)；连夏配方颗粒高、中剂量组大鼠心梗周围区TrKA mR-NA表达均明显降低(P<0．01)；与美托洛尔组比较,连夏配方颗粒高剂量组大鼠心梗周围区NGF mRNA表达明显降低(P<0．05)。结论连夏配方颗粒可显著降低心肌梗死后大鼠心梗周围区心肌组织NGF mRNA、TrKA mRNA的表达,达到改善心肌梗死后大鼠心梗周围区自主神经重构的作用。%Objective To investigate the effects of LianXia Formula Granule ( LXFG) on NGF mRNA、TrKA mRNA expression level at infarcted border zone of rats after myocardial infarction ( MI ) . Methods Ninty healthy male Wistar rats were randomly assigned to six groups: sham operated group, model group, metoprolol group, high, middle and low-dose of LXFG group (189. 00, 94. 50, 47. 25 mg/kg) . The sham operated rats were not ligated in the left anterior descending coronary artery except the rest groups. The sham operated group and model group were given with normal saline by intragastric administra-tion, and the rest groups were given with medicine for testing. 30 days later, NGF mRNA, TrKA mRNA expression level were separately measured in all groups. Results Compared with the model group, NGF mRNA expression level in the high, middle and low-dose of LXFG group decreased (P<0. 01) and TrKA mRNA expression level in the high and middle-dose of LXFG group decreased (P<0. 01). Compared with the metoprolol group, NGF mRNA expression level in the high-dose of LXFG group decreased (P<0. 05). Conclusion Application of LianXia Formula Granule in rats after MI can significantly decrease the expres-sion level of NGF mRNA、TrKA mRNA to improve cardiac autonomic neural remodeling.