甘草地上部分脂溶性总黄酮抗氧化活性研究

摘要

Objective To establish the HPLC fingerprint of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch. , the DPPH radical scavenging, reducing antioxidant power assay, hydroxyl free radical scavenging capacity was used to evaluate antioxidant activity of fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch. . Method HPLC fingerprint of the aerial parts of the fat-soluble flavonoids of Glycyrrhiza uralensis Fisch. was established, HPLC fingerprint chromatographic conditions: DIKMA Wonda Sil®-C18 analytical column (4. 6×250mm,5μm), acetonitrile-0. 1%phosphoric acid - water gradient, the volumetric flow rate was 1mL/min, column temperature was 35℃, detection wavelength was 190 ~400 nm. In vitro antioxidant activity was measured by DPPH radical scavenging method, reducing power and scavenging hydroxyl radical assay. Results Fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch. had a strong antioxidant capacity. Conclusion HPLC-DAD fingerprint method can show fat-soluble flavonoids in aerial parts of Glycyrrhiza uralensis Fisch. . It has good DPPH radical scavenging activity, scavenging hydroxyl free radical activity and reducing power.%目的：建立甘草地上部分脂溶性成分高效液相指纹图谱,采用DPPH自由基清除能力、还原力、羟基自由基清除能力评价甘草地上部分脂溶性总黄酮抗氧化活性。方法建立甘草地上部分脂溶性黄酮类成分高效液相指纹图谱,色谱条件：岛津WondaSil®-C18分析柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸-水溶液梯度洗脱,体积流量1 mL/min,柱温35℃,检测波长190~400 nm。通过体外抗氧化活性法测定甘草地上部分脂溶性总黄酮的清除DPPH自由基能力、还原能力及清除羟基自由基能力。结果甘草地上部分脂溶性黄酮类成分有良好的清除DPPH自由基活性、清除羟基自由基能力及还原能力。结论建立的HPLC-DAD指纹图谱方法能较好的反应甘草地上部分脂溶性黄酮类成分,甘草地上部分脂溶性总黄酮有较强的抗氧化能力。