盆花文心兰丛生芽组培快繁技术研究

摘要

The peduncle axillary buds of Zelglossoda Calico Gem,Green Valley # 1,were used as explants for the study.The bud clumps induction pathway was experimented with orthogonal design to study the effects of the key factors,such as culture medium (i. e., MS, Hyponex # 1, Improvement # 1, Improvement # 2, and Improvement # 3 ),phytohormone (i. e.,6-BA,NAA,and IBA),and CH,on the plant growth during the induction, propagation, and rooting stages. The results showed that the main factors affecting the bud multiplication included 6-BA,basic medium,NAA,and CH.The optimal growth was observed under the use of the combination of Improvement # 1 medium,6-BA 3.0 mg · L-1 ,NAA 0.1 mg · L-1 ,CH 0.5 g · L-1 and agar powder 5.0 g·L-1 to result in an averaged propagation coefficient of 5.8 in 50 days.For the rooting,Improvement #3 with added IBA 0.5 mg · L-1 ,AC 0.5 g · L-1 ,sugar 20 g · L-1 ,agar 3.6 g · L-1 and carrageenan 3.6 g·L-1 was the best with a 100.0% rooting rate.The survival rate of the plantlets in 6 months after transplanting from the test tubes was 96.8%.%以文心兰‘豹斑宝石’花梗为外植体,采用丛生芽诱导途径,利用正交设计法,探讨基本培养基(MS、花宝1号、改良1号、改良2号、改良3号)、植物生长调节剂(6-BA、NAA、IBA)、水解酪蛋白(CH)等对其丛生芽诱导、增殖、生根等关键环节的影响,以期建立文心兰‘豹斑宝石’丛生芽组培快繁技术。结果表明：各试验因素对文心兰丛生芽增殖影响的主次关系为6-BA>基本培养基>NAA>CH；筛选出丛生芽适宜的增殖培养基配方为改良1号+6-BA 3.0 mg·L-1+ NAA 0.1 mg·L-1+CH 0.5 g·L-1+白糖30 g·L-1+琼脂粉5.0 g·L-1,50 d 平均增殖系数达5.8；筛选出适宜生根的培养基配方为改良3号+ IBA 0.5 mg·L-1+活性碳0.5 g·L-1+白糖20 g·L-1+琼脂粉3.6 g·L-1+卡拉胶3.6 g·L-1,生根率为100.0%；试管苗移栽6个月成活率达96.8%。