Formalin killed vaccines with/without white oil adjuvant were prepared with TBY-1 strain of Streptococcus iniae isolated from freshwater fish. The efficacy of the vaccines were determined in Oreochromis niloticus by bath immersion and intraperitoneal (IP) injection successively. The relative percent survival (RPS) of each immune group was statisticsed. Moreover, the length and weight of the tilapias were measured and analyzed by SPSS. The results showed that the RPS of the fish immuned with bath immersion and then immunized intraperitoneally once with none-adjuvant vaccine and white oil adjuvant were 50. 50% and 71. 10% respectively, and the antibody agglutination titer were 1:4. 8 and 1: 128 respectively. The RPS of fish immuned with bath immersion and then immunized intraperitoneally twice with none-adjuvant vaccine and white oil adjuvant were 82. 50% and 58. 80% respectively, and the antibody agglutination titer were 1∶6.4 and 1∶27.4 re spectively.%将海豚链球菌(Streptococcus iniae)淡水分离株TBY-1菌株灭活后制备成无佐剂和添加白油佐剂的疫苗,利用浸泡和腹腔注射复合免疫方式对罗非鱼(Oreochromis niloticus)进行免疫,比较其相对保护率及血清中抗体凝集效价,以评价佐剂的存在与否及免疫次数对海豚链球菌灭活疫苗的免疫效果的影响,同时还对实验前后罗非鱼的体长体重进行测量和统计,并用SPSS软件进行分析.结果显示,用无佐剂疫苗进行首次浸泡免疫后,再分别用无佐剂疫苗及白油佐剂疫苗注射免疫1次的相对保护率分别为50.50％和71.10％,血清中抗体凝集效价分别为1∶4.8和1∶128;而注射免疫2次的相对保护率分别为82.50％和58.80％,其血清凝集效价分别为1∶6.4和1∶27.4.
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