β-环糊精包合法纯化紫苏籽油中的α-亚麻酸

摘要

研究利用微射流均质机强化β-环糊精包合紫苏籽油纯化α-亚麻酸工艺，在单因素试验的基础上，选取用料比、处理压力、包合时间3因素进行Box-Behnken试验设计，并利用响应面法对包合工艺进行优化。优化结果表明，在用料比(紫苏油：β-环糊精)1：11(mL/g)、处理压力6000psi、包合时间105min时，β-环糊精包合紫苏籽油的包合率最高达81.64%。利用FT-NIR、扫描电镜对包合物进行物理特性表征，结果证明β-环糊精能够很好的包合紫苏籽油。GC-MS检测表明包合前后的α-亚麻酸的含量由53.50%提高到77.42%，因此表明β-环糊精包合法纯化紫苏籽油中α-亚麻酸工艺可行。%The preparation of inclusion complexes of Perilla frutescensseed oil withβ-cyclodextrin using a microfluidizer to increase theα-linolenic acid content in the oil was studied. Response surface methodology based on a Box-Behnken design was used to optimizePerilla frutescensseed oil-to-β-cyclodextrin ratio, pressure and inclusion reaction time. The maximum inclusion rate as high as 81.64% was obtained after 105 min of inclusion reaction betweenPerilla frutescens seed oil andβ-cyclodextrin at a ratio of 1:11(mL/g) at 6000 psi. FT-NIR analysis and SEM observation showed thatPerilla frutescens seed oil was wel included byβ-cyclodextrin in the inclusion complex obtained. Theα-linolenic acid content increased from 53.50% to 77.42% afterβ-cyclodextrin inclusion as shown by HPLC analysis. Therefore,β-cyclodextrin inclusion is a feasible way to concentrate theα-linolenic acid content inPerilla frutescensseed oil.