荔枝皮原花青素与VC、VE的协同抗氧化研究

摘要

选取荔枝皮原花青素（LPPC）,采用DPPH自由基清除实验与等辐射分析法（Isobologram）相结合,探讨荔枝皮原花青素、VC、VE单独和复配后的抗氧化作用,结果显示：荔枝皮原花青素、VC和VE的IC5 0值分别为9.98、9.21、27.96mg/L,表明荔枝皮原花青素对DPPH自由基有明显的清除作用。Isobologram分析图中荔枝皮原花青素与VC、VE复配后的效应点都在相加线及95%可信限的下方,理论IC50add值与实验IC50mix值有显著性差异,并且相互作用指数都小于1,证实荔枝皮原花青素与VC,荔枝皮原花青素与VE之间存在协同抗氧化效应。%In order to explore the synergistic antioxidant effects of procyanidins from Litchi chinensis pericarp（LPPC） and vitamin C（VC） or vitamin E（VE）,the antioxidant capacity of LPPC in the presence of VC or VE was evaluated by DPPH radical scavenging assay coupled with isobologram method.The results indicated that the IC50 of LPPC,VC and VE were 9.98,9.21mg/L,and 27.96 mg/L,respectively,which suggested that LPPC had significant DPPH radical scavenging effect.The effect-response points of LPPC combined with VC or VE were observed below the addition line with 95% confidence interval in isobolographic plots.Meanwhile,a significant difference between theoretical IC50add value and experimental IC50mix value was achieved.The interaction index of each combination was less than 1.These findings indicate that there is a synergistic antioxidant effect between LPPC and VC or VE,which will reveals promising prospects in practical applications.