镰刀菌（Fusarium sp.B158）色素的提取及其理化性质

摘要

Abstract： In this work, pigments from the liquid-cultured mycelia of Fusarium sp. B158 were extracted and their physico-chemical properties were investigated. The best solvent for extracting pigments from the liquid-cultured mycelia of Fusarium sp. B158 was found to be acidified ethanol （pH = 2）. The extract obtained showed maximum absorption at 324 nm and 360 nm. To facilitate further investigations, 360 nm was chosen as maximum absorption wavelength. The pigments were stable to heat, light, sugars and common food additives, but sensitive to pH, oxidizers and reductants. Most of the investigated metal ions （except Cu2＋ and Fe3＋） revealed little effect on the pigments.%从一株新型镰刀菌（Fusarium sp.B158）液态发酵菌丝体提取色素,并对其理化性质进行研究。结果表明：该色素的最佳提取溶剂是酸性乙醇（pH2）。色素的全波长扫描显示,该色素在324nm和360nm波长处均有吸收峰,实验,选择360nm作为色素的最大检测波长。色素的稳定性研究表明,色素对热、光、甜味剂以及常见食品添加剂表现稳定,对溶剂的pH值以及氧化剂和还原剂敏感。大部分的金属离子（Cu2＋和Fe3＋除外）对色素的稳定性影响不大。