联合补充维生素D、胶原肽和钙对成骨细胞发育的影响

摘要

目的:观察联合补充维生素D、胶原肽和钙对MC3T3-E1成骨细胞增殖及对骨保护素细胞内核因子κB受体活化因子配体(receptor activator of NF-κB ligand,RANKL)、骨保护素(Osteoprotegerin,OPG)基因表达的影响.方法:α-MEM培养基培养MC3T3-E1细胞.检测Ca2+(20 μg/mL)、维生素D(0、10-12、10-11 mol/L)、胶原肽(0、50、100μg/mL)三者交互作用剂量对MC3T3-E1细胞增殖的作用及RANKL、OPG mRNA表达.结果:维生素D、胶原肽和钙交互剂量作用下细胞增殖水平无明显差异.维生素D联合钙能够明显降低RANKL mRNA表达水平,提高OPG mRNA表达水平,降低RANKL/OPG比值.而胶原肽联合钙对RANKL以及OPG mRNA表达无明显影响.结论:维生素D联合钙可通过抑制小鼠成骨细胞RANKL mRNA表达、促进OPG mRNA表达,从而促进骨的形成,抑制骨的吸收.维生素D和钙联合补充胶原肽,对成骨细胞RANKL,OPG mRNA表达并无明显影响.%[Objective] To explore the effect of combined supplementation of vitamin D,collagen peptides and calcium on the proliferation and expression of RANKL and OPG gene in MC3T3-E1 cells.[Method] MC3T3-E1 osteoblasts were cultured with α-MEM.The effect of interaction intervention of Ca2+ (20 μg/mL),vitamin D (0 mol/L,10-12mol/L,10-11mol/L),CP (0 μg/mL,50 μg/mL,100 μg/ mL) on the proliferation of MC3T3-El osteoblast and the RANKL and OPG mRNA expression were assessed.[Result] Proliferation rates of MC3T3-E1 cells were not significantly different by the interaction intervention of vitamin D,CP and Ca.It showed markedly decreased ltevels of mRNA expression for RANKL,increased mRNA expression for OPG and significantly decreased RANKL/OPG mRNA ratio by the intervention of vitamin D combined with Ca.However,collagen peptides with Ca played no obviously effect on the expression of RANKL and OPG mRNA.[Conclusion] Vitamin D combined with Ca may promote bone formation and inhibit bone absorption by decrease RANKL mRNA expression and increase OPG mRNA expression.However,combined CP with vitamin D and Ca plays no significant role on the expression of RANKL and OPG mRNA expression in osteoblasts.