嗜酸乳杆菌培养基的优化及高密度培养

摘要

The optimal medium of Lactobacillus acidophilus was studied by single factor experiments and orthogonal experiments. The results showed that the optimum medium formula was 2.0％ sucrose; 1.5％ pepton; 0.5％ yeast extract;0.2％ KH2PO4,optimal initial pH 5.9; optimal growth promoting factors 5％ tomato juice. The cell density of the optimum medium was 9.3×108CFU/mL in 15L auto-fermentator and 9.0×108CFU/mL in 2M3fermentation tank.%针对益生菌嗜酸乳杆菌的营养需要,优化其增殖培养基,获得其高密度培养条件.采用单因素试验和正交试验,时嗜酸乳杆菌增殖培养基进行优化.单因素试验结果表明:最佳碳源、氮源、磷源分别为蔗糖、蛋白胨、酵母膏、KH2PO4;正交试验确定的最佳添加量为蔗糖2.0%、蛋白胨1.5%、酵母膏0.5%、KH2PO4 0.2%.最佳初始pH为5.9;最佳增殖因子为5%西红柿汁.优化培养基的菌体浓度为9.3×10℃FU/mL,是基础培养基的5.8倍;2M3发酵罐扩大培养的菌体浓度达9.0×10℃FU/mL,终点pH为3.8.