恒速补料与pH胁迫相结合提高透明质酸发酵产量的研究

摘要

通过对兽疫链球菌恒速补料培养与间歇性pH胁迫相组合提高透明质酸发酵产量的研究，发现通过恒速补料培养模式与间歇性的pH胁迫相结合的方式，能使菌体获得较高的菌体比生长速率与碳源利用率，同时转变细胞产能途径、提高细胞产能效率，有效提高HA 的发酵产量。该研究最终得到HA 的发酵产量为6.724g/L，比单一恒速补料培养产量提高了56.4%；比单一间歇性pH胁迫培养产量提高了25.8%，比对照提高了68.1%。%The Streptococcus zooepidemicus constant fed-batch culture and intermittent pH stress combination of hyaluronic acid fermentation yield, through constant batch culture mode and intermittent pH stress combination, can make the cell to obtain a higher specific cell growth rate and carbon source utilization rate, at the same time change the cell capacity approach, improve cell production efficiency, improve the yield of fermentation HA. The study finally obtained the fermentation yield of HA was 6.724g/L, than a single constant fed-batch production increased by 56.4%; pH stress than single intermittent training of production increased by 25.8%,increased by 68.1% than that of contrast.