γ-聚谷氨酸高产菌株的诱变选育研究

摘要

本文对一株产γ-聚谷氨酸的地衣芽孢杆菌（Bacillus licheniformis）进行了紫外照射、紫外-硫酸二乙酯、紫外-亚硝基胍复合诱变筛选研究以及诱变株的摇瓶筛选研究，建立了聚谷氨酸产生菌地衣芽孢杆菌最佳诱变方法：紫外诱变8min，致死率为90.6％；紫外-硫酸二乙酯复合诱变致死率94%；紫外-亚硝基胍复合诱变致死率89.8%诱变效果最好。诱变株γ-聚谷氨酸产率最高达到了3.55%，比出发菌株1.48%提高了140%。%In this paper, a strain producting γ-polyglutamic acid Bacillus licheniformis was studied by UV irradia-tion, UV-Diethyl sulfate (UV-DES), UV-Nitrosoguanidine (UV-NTG) Compound mutation screening and shake flask screening. The optimum mutation method for γ-polyglutamic acid producting strain Bacillus licheniformis was estab-lished: UV mutagenesis of 8min, mortality 90.6%; UV-DES compound mutation mortality 94%; UV-NTG mortality 89.8%. The mutant γ-poly glutamic lutamic acid yield up to 3.55%, 140% higher than the original strain 1.48%.