蓝莓叶水溶性和醇溶性提取物的抗氧化作用

摘要

Aqueous and ethanolic extracts were extracted from blueberry leaves. Anti-lipid peroxidation, reducing power, scavenging hydroxyl radicals, superoxide anion radical and DPPH radical of aqueous and ethanolic extracts from blueberry leaves were determinated by in vitro chemical system. The results showed that aqueous and ethanolic extracts from blueberry leaves strongly inhibited lipid peroxidation, enhanced reduction power, scavenged hydroxyl radical, superoxide anion radical and DPPH· radical. Aqueous and ethanolic extracts from blueberry leaves were enhanced on anti-lipid peroxidation, reducing power, scavenging free radical activity with increasing their concen-tration. Ethanolic extracts were stronger than aqueous extracts on anti-lipid peroxidation ability , whereas they were weaker than aqueous extracts on scavenging hydroxyl radical, superoxide anion radical and DPPH radical. There was not any significant difference between them on reduction power. Blueberry leaves extracts have characteristics of antioxidant effect on lipid peroxidation and free radical and it can be applied as an effective natural antioxidant.%以蓝莓叶为原料，提取了其中的水溶性和醇溶性物质，通过体外化学模拟体系，测定了蓝莓叶水溶性提取物和醇溶性提取物的抗脂质体过氧化、还原力、清除羟自由基、超氧阴离子自由基、DPPH·自由基的能力。结果表明水溶性提取物和醇溶性提取物均具有抗脂质体过氧化能力、增强还原力、清除羟自由基(·OH)、超氧阴离子自由基(O2-·)﹑DPPH·自由基的能力，并且随浓度的增加抗脂质过氧化、还原力、清除自由基能力增强。醇溶性提取物抗脂质过氧化能力强于水溶性提取物，但水溶性提取物清除羟自由基、超氧阴离子自由基、DPPH·自由基能力强于醇溶性提取物，二者还原力无显著性差异。蓝莓叶提取物具有明显的抗脂质体过氧化作用和清除自由基能力，可作为有效的天然抗氧化剂。