固态发酵制备富含纳豆激酶豆渣的工艺优化

摘要

本试验通过单因素试验和正交试验,以豆渣纳豆激酶活性为指标,就固态发酵工艺参数进行优化.试验结果表明,最适的发酵条件为培养基初始含水量65%,培养基初始pH 7,发酵温度35℃,接种量4%,发酵时间28h,此条件下通过固态发酵得到的豆渣中纳豆激酶活性为1734U/g.%The best fermentation parameters for obtaining nattokinase-containing okara were determined by single-factor experiment and orthogonal test. The results showed that the optimum solid fermentation parameters were as followed: the initial water amount was 65%, the pH was 7, the temperature was 35℃, the inoculation content was 4.0% and the fermentation duration time was 28h. With these conditions, the activity of okara nattokinase ob-tained by solid fermentation was 1734U/g.