蛋白粉中转基因成分实时荧光定量PCR检测方法的建立

摘要

建立了应用实时荧光定量PCR技术检测蛋白粉中转基因成分的方法.通过提取DNA,优化时荧光定量PCR反应体系条件,建立了一种简便快速的检测蛋白粉中转基因成分的方法,具有良好特异性和灵敏度,检出限为0.05%.该方法检测条件稳定,样品前处理简单,是一种快速简便、可靠的方法,适合于推广使用.%A method for the detection of genetically modified components in protein powder by real-time PCRwas established.Optimization of real-time PCR reaction system conditions by extracting DNA. A simple and rapid method for the detection of genetically components in protein powder was established, with a good specificity and sensitivity with a detection limit of 0.05%. The method has the advantages of stable detection conditions, simple sample preparation, quick and easy method and reliable method, and is suitable for popularization and use.