Comparative Studies of the Carbohydrate of Human Gamma-Crystallins from Fetal and Adult Lenses with Agglutinins

摘要

Using gel chromatography of Sephadex G-75 superfine connectedwith Sephadex G-50 fine column,three human γ- crystallins(γ1,γ2,γ3)couldbe obtained.Seven agglutinins(LCA,SBA,DBA,PNA,BSL,RCA and UEA)were used to detect the sugar of sub-γ-crystallins,which had been transferredto nitrocellulose membrane and finally stained with ABC reagents and the sub-strate of HPR.These results suggested that γ2-and γ3-crystallin contain sugar,but γ1-crystallin has no sugar.There is a decrease of carbohydrate of γ2 and γ3as...