高效液相色谱法测定升血小板胶囊中靛玉红的含量

摘要

目的:建立升血小板胶囊中靛玉红的含量测定方法.方法:采用高效液相色谱法测定升血小板胶囊中靛玉红的含量.色谱柱为依利特ODS-C18柱(150 mm×4.6 mm,5μm);流动相为甲醇-1％甲酸溶液(V:V=30:70);柱温为30℃;流速为0.8 ml/min;检测波长为289 nm.结果:靛玉红质量浓度在0.20～5.08μg/ml范围内线性关系良好(r=0.9999);精密度、稳定性和重复性试验的RSD均<2％;样品加样回收率为98.97％～100.92％,RSD均<2％.结论:本方法简便、灵敏及准确,专属性强,可作为升血小板胶囊的含量测定方法.%OBJECTIVE:To establish method for content determination of indirubin in Shengxuexiaoban capsules . METHODS:High performance liquid chromatography ( HPLC ) was adopted to detect the content of indirubin in Shengxuexiaoban capsules .The ODS-C18 ( 150 mm ×4.6 mm, 5 μm ) was set as chromatographic column; mobile phase was methanol-1％of formic acid ( V:V=30:70 ); column temperature was 30 ℃; flow rate was 0.8 ml/min;detection wavelength was 289 nm.RESULTS: The linear relation remained well ( r =0.9999 ) when the mass concentration of indirubin stayed within 0.20-5.08 μg/ml; all the relative standard deviations ( RSD ) of precision degree, stability and repetitive test were <2％; the recovery rate of sample addition was 98.97％-100.92％, with RSD<2％.CONCLUSIONS:This HPLC method is convenient , sensitive and accurate , with strong specificity , which can be served as the method for content determination of Shengxuexiaoban capsules .