类肝素聚乳酸微孔膜的血液相容性

摘要

通过在生物基聚乳酸(PLA)膜表面进行类肝素化修饰,得到了具有良好血液相容性的生物基聚合物膜.首先通过自由基聚合合成了聚(乙烯基吡咯烷酮 – 乙烯基三乙氧基硅烷)[P(VP–VTES)]预聚物,进一步通过其界面交联,将高含量的聚乙烯基吡咯烷酮固化到PLA膜表面,提高膜表面的亲水性和抗污性能.其次通过自由基聚合合成类肝素预聚物苯乙烯磺酸钠 – 乙烯基三乙氧基硅烷 – 丙烯酸三元共聚物[P(SSNa–VTES–AAH)],并利用预聚物中VTES链段的水解缩合反应将类肝素共聚物固定在PLA膜表面,从而提高膜的血液相容性.通过红外光谱确定了PLA膜表面亲水层P(VP–VTES)和P(SSNa–VTES–AAH)的分子结构.通过接触角测试表明类肝素分子修饰的PLA膜具有良好的亲水性,并可以抑制膜表面血小板的吸附和聚集.同时,凝血测试结果表明类肝素分子修饰的PLA膜表面具有良好的血液相容性.%PLA microporous membrane decorated by heparin-mimicking polymer with good hemocompatibility was prepared. P(VP–VTES) was immobilized on the PLA membrane surface via the interfacial crosslinking method to enhance the hydrophilicity and fouling resistance. Heparin-mimicking prepolymer P(SSNa–VTES–AAH) was synthesized by free radical polymerization and then immobilized on the membrane surface via the hydrolysis and condensation. ATR–FTIR was used to confirm the chemistry variation of the membrane surface. Contact angle measurements indicate that the modified membrane possesses excellent hydrophilicity,which inhibites the adsorption and aggregation of platelets. The blood concretion items show that heparin-mimicking surface has good hemocompatibility.