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RASSF1AMethylation Status and BRAF V600E Immunohistochemical Expression in Odontogenic Lesions

机译:牙源性病变中的RASSF1AM甲基化状态和BRAF V600E免疫组织化学表达

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摘要

Background: The etiology and pathogenesis of odontogenic lesions remain to be determined. Previous studies have identified epigenetic and genetic alterations that may be relevant to lesions progression and development. Hypermethylation of the Ras association domain family protein 1A (RASSF1A) has been observed in a variety of human cancers. However, the methylation status of RASSF1A in odontogenic lesions remains unknown. Thus, the aim of this study was to investigate the prevalence of RASSFA promoter hypermethylation and v-raf murine sarcoma viral oncogene homolog B V600E mutant (BRAF V600E) expression as well as the correlations between these alterations and clinicopathological features of patients with odontogenic lesions. Methods: We subjected 66 formalin-fixed, paraffin-embedded odontogenic lesions [ameloblastoma (AM), 21;ameloblastic carcinoma (AC), 6;odontogenic keratocyst (OKC), 19;and dentigerous cyst (DC), 20] to methylation-specific polymerase chain reaction to determine RASSF1A hypermethylation and immunohistochemistry to detect BRAF V600E protein expression. Results: We observed RASSF1A hypermethylation in 20% (4/20;methylation could not be detected in one lesion), 100% (6/6), 26.3% (5/19), and 5% (1/20) of AM, AC, OKC, and DC samples, respectively. RASSF1A methylation was significantly more frequently observed in AC relative to AM, OKC, and DC (p Conclusions: Concomitant RASSF1A methylation and positive BRAF V600E expression are commonly observed in AC, which may contribute to AC tumorigenesis.
机译:背景:依然测定牙突病变的病因和发病机制仍有待确定。以前的研究已经确定了与病变进展和发育有关的表观遗传和遗传改变。在各种人类癌症中已经观察到RAS结合域系蛋白1A(RASSF1A)的高甲基化。然而,渡齿菌病变中Rassf1a的甲基化状态仍然未知。因此,本研究的目的是研究RASSFA启动子高甲基化和V-RAF鼠Sarcoma病毒癌基因同源物(BRAF V600E)表达的患病率以及牙肠病病变患者的这些改变和临床病理特征之间的相关性。方法:我们进行66例福尔马林固定的石蜡包埋的牙科病变[ameloblastoma(am),21;含氨基细胞癌(AC),6; odontogenic角蛋白(OKC),19;和直接囊肿(DC),20]甲基化 - 特异性聚合酶链反应,以确定RASSF1A高甲基化和免疫组织化学检测BRAF V600E蛋白表达。结果:我们观察到rassf1a高甲基化20%(4/20;不能在一个病变中未检测到甲基化),100%(6/6),26.3%(5/19)和5%(1/20) ,AC,OKC和DC样品。在AC,OKC和DC相对于AM,OKC和DC中,RASSF1A甲基化显着观察到(P:Conclyapant Rassf1A甲基化和阳性BRAF V600E表达,在AC中通常观察到,这可能有助于AC肿瘤瘤。

著录项

  • 来源
    《病理学期刊(英文)》 |2020年第003期|P.93-107|共15页
  • 作者单位

    Division of Oral Pathology Department of Health Promotion Kyushu Dental University Kitakyushu JapanDepartment of Oral Surgery and Oral Medicine Faculty of Dentistry Srinakharinwirot University Bangkok Thailand;

    Division of Oral Pathology Department of Health Promotion Kyushu Dental University Kitakyushu Japan;

    Division of Oral Pathology Department of Health Promotion Kyushu Dental University Kitakyushu Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肿瘤学;
  • 关键词

    Odontogenic Lesions; DNA Methylation; RASSF1A; Immunohistochemistry; BRAF V600E;

    机译:牙源性病变;DNA甲基化;RASSF1A;免疫组织化学;BRAF V600E;
  • 入库时间 2022-08-19 04:46:18
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