Study of a Whitening and Anti-wrinkle Bio-functional Ingredient Based on Epigenetics

摘要

Via quantitative polymerase chain reaction (qPCR) test method,the efficacy of hexapeptide-2 was studied,including silencing on miR-29 and increasing miR-218 expression which targeted collagen coding mRNA (message RNA) then decreases the collagen synthesis,and the latter silences the melanin synthesis related enzyme.Then the double blind spilt-face clinical test on 35 Asian people (18 male and 17 female) was conducted to evaluate the whitening and anti-wrinkle effect.The qPCR test showed that hexapeptide-2 can decrease miR-29 expression for human fibroblast of different age (P ＜ 0.05);and the result on human melanocyte showed that it can increase miR-218 expression (P ＜ 0.05).Immunostaining results showed that it can increase the expression of collagen Ⅰ and Ⅲ significantly (P ＜ 0.05).The double blind split-face test showed that 1％ hexapeptide-2 displays significant efficacy for skin whitening,spots lightening,skin erythema and skin dullness improvement,as well as skin wrinkles reduction as comparing with that from the placebo side (P ＜ 0.05).