Protocols for Pachytene Chromosome and DNA Fiber FISH in Cotton

摘要

@@ Fluorescence in situ hybridization (FISH) has become the most important technique in plant molecular cytogenetics research.FISH-based physical mapping provides a valuable complementary approach in genome sequeneing to measure the physical distances between adjacent BAC contigs and delineating the structure and DNA composition of genomic regions of centromere and telomere.The accuracy and precision of the FISH-base physical maps depend on the resolution power of FISH.