碘促进血管内皮细胞增殖作用与MEK1相关性及碘对血管内皮细胞表达VEGF影响的实验研究

摘要

Objective To study the mechanism of iodine on proliferation activity of Vascular endothelial cells(VEC). Methods 1.VEC were cultured in iodine and treated with mitogen-activated protein kinase 1 (MEK1) inhibitor then examined the VEC proliferation activity by CCK-8 assy. 2.VEC were cultured in different doses iodine (100-300μg/L) and used Western-blot and RT-PCR to measure the VEGF expression. Results VEC cultured in iodine and treated with MEK1 inhibitor did not excessively proliferate infuenced(P<0.01).The expression of VEGF did not indicate an obvious increase in different iodine group(P>0.05).Conclusion Iodine to facilitate proliferation of VEC related the activation of MEK1. Iodine did not promote VEGF expressing VEGF protein.%　　目的研究碘促进血管内皮细胞(VEC)增殖的机制.方法(1)使用丝裂原活化蛋白激酶激酶1(MEK1)抑制剂作用与含有碘离子(I-)培养环境中的VEC,以CCK-8法检测细胞增殖情况；(2)在不同浓度环境中培养VEC,使用免疫印迹法和RT-PCR法检测血管内皮生长因子(VEGF)表达情况.结果MEK1抑制剂作用于含有I-培养环境中的VEC后,细胞相对增殖率低于KI组(P<0.01).不同浓度的I-作用于VEC后,VEGF表达未出现上调(P>0.05).结论碘促进血管内皮细胞增殖可能与MEK1激活有关,碘不促进VEC合成VEGF.