对异甘草素的合成工艺进行优化和体外清除自由基活性进行研究.通过间苯二酚、冰醋酸、对羟基苯甲酸合成异甘草素,考察物料比、温度、反应时间对产率的影响,通过紫外分光度法对其清除自由基活性进行研究.酚与酸物料比1:2.5,240 W微波辐射3 min,2,4-二羟基苯乙酮产率最高为65.75%;当醛酮物质的量比为3:1,温度控制在120~130℃,回流6 h,异甘草素产率最高为28.10%;反应时间控制20 min,异甘草素加入量为1.00 mL清除DPPH自由基能力最强,为66.4%.异甘草素具有一定的抗自由基活性.%The synthesis process of isoliquiritigenin was optimized and its activity of scavenging free radicals in vitro was studied. Firstly, isoliquiritigenin was prepared from resorcinol, acetic acid and p-hydroxybenzoic acid. The influence of molar ratio of reactants, reaction temperature and reaction time on the yield of isoliquiritigenin product was investigated. The results showed that, when the molar ratio of resorcinol and acetic acid was 1:2.5, microwave radiation was 240 W for 3 min, the product yield of 2, 4-dihydroxyacetophenone reached the maximum value, 65.75%; When the molar ratio of aldehyde and ketone was 3:1, the temperature was about 120~ 130 oC with reflux time of 6 h, the yield of isoliquiritigenin product was as high as 28.10%. Then, the activity of scavenging DPPH free radicals was the highest, about 66.4 when 1.00 mL isoliquiritigenin was added to the solution with the reaction time of about 20 min. The results indicated that isoliquiritigenin has good anti-free radicals ability.
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