目的 探究异甘草素对大鼠麻醉手术后学习记忆能力及海马组织氧化物的影响.方法 将40只SD雄性大鼠随机分为对照组(C组)、手术组(P组)、异甘草素小剂量组(T1组)和异甘草素大剂量组(T2组) ,每组10只. P组腹腔注射75 mg/kg氯胺酮+5 mg/kg咪达唑仑进行麻醉,并行剖腹探查术;T1组给予10 ml/kg异甘草素灌胃60 min后开始麻醉,T2组给予20 ml/kg异甘草素灌胃60 min后开始麻醉,麻醉及手术操作同P组;C组腹腔注射与P组等容的0. 9%氯化钠溶液. P组、T1组和T2组大鼠清醒3 h后,C组腹腔注射0. 9%氯化钠溶液后3 h行水迷宫实验评价各组学习记忆能力;继之处死大鼠取双侧海马组织测定各组丙二醛( MDA) 、超氧化物歧化酶( SOD) 、谷胱甘肽( GSH)水平.结果 与C组相比,P组逃避潜伏时间明显延长,目标象限停留时间明显缩短,MDA 明显增加,SOD 和 GSH 均明显降低,差异均有统计学意义( P <0. 01);与P组相比,T1组与T2组逃避潜伏时间明显缩短,目标象限停留时间明显延长,MDA明显降低,SOD和GSH均明显增加,差异均有统计学意义(P<0. 01);T1组与T2组相比逃避潜伏时间延长,目标象限停留时 间缩短,MDA明显增加,SOD和GSH均明显降低,差异亦有统计学意义( P<0. 05或P<0. 01) .结论 异甘草素能够有效改善大鼠麻醉手术后学习记忆能力,其作用机制可能与抗脂质过氧化有关.%Objective To investigate the effect of isoliquiritigenin on learning and memory ability as well as on oxides of hippocampal tissues of rats after operation. Methods Forty male Sprague-Dawley rats were randomly divided into 4 groups: control group(group C, n＝10), operation group(group P,n＝10), low-dose group(group T1, n＝10) and high-dose group(group T2, n＝10). Group P received intraperitoneal injection of 75 mg/kg ket-amine and 5 mg/kg midazolam for anesthesia,and then exploratory laparotomy was performed. Group T1 and T2 were given 10 ml/kg and 20 ml/kg isoliquiritigenin respectively for gastric perfusion, followed by anesthesia after 60 min. Anesthesia and operation were performed as described above in group P. Group C was injected intraperitoneally with the normal saline of the same volume as group P. Cognitive function including learning and memory ability was as-sessed using Morris water maze test 3 h after rats in group P, group T1 and group T2 regained consciousness and 3 h after group C received intraperitoneal injection of 0. 9% sodium chloride solution. Rats were then sacrificed to collect bilateral hippocampal tissues, and the expressions of malondialdehyde( MDA) and superoxide dismutase ( SOD) and glutathione ( GSH) in hippocampus were measured. Results Compared with group C, the latency time was signifi-cantly prolonged and the duration of the target quadrant was significantly shortened in group P. In addition, the ex-pression of MDA was increased significantly, whereas SOD and GSH were decreased ( P<0. 01) . Compared with group P,the latency time was significantly shortened and the duration of the target quadrant was significantly pro-longed in group T1 and T2. Moreover, there was remarkably decreased expression of MDA and notably increased SOD and GSH ( P<0. 01) . Compared with group T2, the latency time was significantly prolonged and the duration of the target quadrant was significantly shortened in group T1. Additionally, the expression of MDA was increased significantly, whereas SOD and GSH were decreased (P<0. 05 or P<0. 01). Conclusion Isoliquiritigenin can ef-fectively improve the ability of learning and memory of rats after operation, the mechanism of which may be related to the inhibition of anti-lipid peroxidation.
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