大黄素对脓毒症急性肺损伤大鼠水通道蛋白1表达的影响

摘要

Objective To investigate the effects of emodin on aquaporin 1 (AQP-1 )expression in acute lung injury in sepsis rats.Methods A total of 120 adult SD rats weighing 200-230 g were randomly divided into six groups, blank control group,sham group,model group,low dose emodin pretreatment group,middle dose emodin pretreatment group,high dose emodin pretreatment group,20 rats in each group.The rats with sepsis were induced by cecal ligation and puncture(CLP).The abdominal cavity in sham group was closed after cecal exploration operation.Each group was further divided into four time points:3 hours,6 hours,12 hours and 24 hours after the modeling,with 5 rats in each time point,according to the specimen collection time.Rats were sacrificed at different time points to measure the expression of AQP-1 mRNA by real-time fluorescence quantitative PCR and AQP-1 protein by western-blot.Results AQP-1 expression at 3 h among different groups showed no significant difference.After modeling,AQP-1 at 6 h decreased gradually and reached the minimum 1 2 h.AQP-1 of the middle and high dose emodin pretreatment groups at 12 h were significantly higher than those of the same dose of other time points(P <0.01).There was no significant difference between the two groups(P >0.05).Conclusion Sepsis caused the AQP-1 expression of rats significantly lower.Early prophylactic use of middle or high dose emodin can significantly increase AQP-1 expression.%目的：探讨大黄素对脓毒症急性肺损伤大鼠水通道蛋白1(AQP-1)表达的影响。方法成年雄性 SD大鼠120只,体质量200~230 g。大鼠分为6组：空白对照组、假手术组、模型组、大黄素低剂量预处理组、大黄素中剂量预处理组、大黄素高剂量预处理组,每组各20只。采用盲肠结扎穿孔法复制脓毒症大鼠模型,假手术组探查取出盲肠后还纳腹腔。各组按取材时间不同又分为造模后3、6、12、24小时4个时相点,每个时相点5只大鼠。分别用实时荧光多聚酶链反应(PCR)和蛋白免疫电泳(western-blot)法检测肺组织水通道蛋白1信使 RNA(AQP-1 mRNA)和蛋白的表达。结果各组3小时AQP-1表达差异无统计学意义,造模后6小时AQP-1逐渐降低,12小时达最低。大黄素中、高剂量预处理组12小时AQP-1均显著高于同剂量其他时间点(P<0.01),且两组之间差异无统计学意义(P>0.05)。结论脓毒症可造成大鼠 AQP-1表达明显降低,早期预防性使用中高剂量大黄素可明显提高 AQP-1的表达,从而有效减轻脓毒症大鼠肺水肿的程度。