目的 探讨贵州省及周边地区牛群博尔纳病病毒(BDV)感染状况.方法 采用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)检测了120例牛的外周血单个核细胞中的BDV p24基因片段.结果 牛外周血单个核细胞(PBMCs)中BDV p24基因片段阳性率均为4.17%.牛BDV p24基因片段的测序结果与GeneBank提供的标准病毒株比较,同源性96.51%～97.67%;有2个位点出现一致性沉寂突变(nt1675 C-T,nt1678 T-C突变率为2%);与马的Strain V病毒株比较,有3个位点出现一致性沉寂突变(nt1650 T-C,nt1671 C-T,nt1674 C-T突变率为3%);与H1766病毒株比较,有2个位点出现一致性沉寂突变(nt1675 C-T,nt1678 T-C突变率为2%);与He80/FR病毒株比较,在3个位点出现一致性沉寂突变(nt 1660 T-C,nt 1669 A-G,nt 1672 C-T突变率为3%);但它们所编码的氨基酸没有改变.结论 贵州及湖南省部分地区牛群中存在BDV自然感染,可能是BDV流行区域之一;人感染BDV可能具有潜在的动物源性.%Objective To investigate the infection condition in cattle infected with Borna disease virus(BDV)in Guizhou province and some surrounding areas. Methods The p24 gene fragment of BDV-RNA in peripheral blood mononuclear cells(PBMCs)from 120 cattle was detected by fluorescence quantitative nested reverse transcriptase polymerase chain reaction(FQ-nRT-PCR). Results The p24 gene fragment of BDV-RNA in PBMCs from cattle was 5 positive(4. 17%). The homology of p24 gene fragment of BDV detected to GenBank was 96. 51% - 97. 67%. 2 situs consistency silent mutation when compared with Bo/04W and huP2br isolated in heifer brain tissue by Watanabe Y(ntl675 C-T,ntl678 T-C mutation rate of 2%). 3 situs consistency silent mutation when compared with Strain V in horse (ntl650 T-C,ntl671 C-T,ntl674 C-T mutation rate of 3%). 2 situs consistency silent mutation when compared with H1766 in horse (ntl675 C-T,ntl678 T-C mutation rate of 2%). 3 situs consistency silent mutation when compared with He80/FR (nt 1660 T-C,nt 1669 A-G,nt 1672 C-T mutation rate of 3%). However,there was no change in the encoding amino acid residues. Conclusion There is BDV natural infection probably originated from cattle of Gui Zhou and surrounding areas,also probably is one of the prevalence regions of BDV. BDV might play a potential role in the development of human having correlations with animals.
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