IL-10对脑出血大鼠HSP70、caspase-3表达的影响

摘要

目的 观察白细胞介素10(IL-10)对出血性颅脑损伤大鼠脑组织热休克蛋白70(HSP70)、胱天蛋白酶3(caspase-3)表达的影响,以探讨IL-10对出血性颅脑损伤的保护作用及机制.方法 96只SD大鼠随机分为正常组(N组)、模型组(M组)、IL-10低剂量组(L组)、IL-10高剂量组(H组).Ⅶ型胶原酶注射法制备颅脑损伤模型,记录各组6 h、12 h、1 d、3 d、7 d大鼠神经功能改善程度;免疫组化法测定HSP70和caspase-3的表达.结果 与M组比较,L组和H组HSP70表达明显升高(P<0.01),caspase-3表达明显降低(P<0.01),神经功能缺损评分明显下降(P<0.01),且以H组更为显著(P<0.05).结论 IL-10可通过上调HSP70表达及抑制caspase-3表达对出血性颅脑损伤起保护作用.%Objective To observe the effects of IL-10 on expression of HSP70 and caspase-3 after intracerebral hemorrhage in rats and study the neuroprotection mechanism of IL-10 for intracerebral hemorrhage. Methods 96 SD rats were randomly divided into four groups:normal group(group N),model group(group M) , Low-dose IL-10 treatment group(group L) and high-dose IL-10 treatment group(group H). The intracerebral hemorrhage rat model was established by intracranial injection of Ⅶcollagenase. The recovery of rats' neurological function of all groups was recorded after 6 h,12 h,l d,3 d,7 d;The expressions of HSP70 and caspase-3 were analyzed by immunohistochemistry. Results The expression of HSP70 in perihematoma in group L and H were up-regulated(P<0. 01) , caspase-3 and the score of neurologic impairment were downregulated as compared to M group(P<0. 01). The above roles of IL-10 were more significant in group H(P<0. 05). Conclusion IL-10 plays protective role in hemorrhagic brain damage by upregulation of HSP70 and downregulation of caspases-3.