电针结合重复经颅磁刺激对焦虑模型大鼠中枢肾上腺皮质激素受体基因表达的影响

摘要

Objective To explore the effects and partial mechanisms of centrum by electroacupuncture combined with repetitive transcranial magnetic stimulation (rTMS) in anxiety rat model. Methods The anxiety rat model was built by SPS, which was used to observe the anti-anxiety effects of electroacapuncture combined with rTMS. The gene expressions of MR and GR in the hippocampus and the prefrontal cortex were measured by RT-PCR. Results Emotional reaction in anxiety model could be reduced by three of therapeutics. Compared with electroacupuncture treatment and rTMS treatment, electroacupuncture combined with rTMS treatment showed more dominance. Compared with the model group,the gene expressions of GR in the hippocampus was significant increased in electroacupuncture combined with rTMS. Conclusion There is a cooperativity of anti-anxiety in the electric acupuncture combined with rTMS. Anti-anxiety effects of therapeutics are possibly related to modulation of the gene expressions of GR in the hippocampus.%目的 研究电针结合重复经颅磁刺激(rTMS)抗焦虑效应的中枢部分作用机制.方法 以单个持续刺激(SPS)方法复制大鼠焦虑动物模型,观察电针结合rTMS抗焦虑效应,运用逆转录-聚合酶链反应(RT-PCR)法检测大鼠海马和前额皮质区的盐皮质激素受体(MR)和糖皮质激素受体(GR)的基因表达.结果 电针结合rTMS具有抗焦虑叠加效应,还可升高焦虑模型大鼠海马GR基因表达水平.结论 电针结合rTMS具有抗焦虑协同效应,其机制可能与调节海马GR基因表达有关.