蒙脱石联合甘露醇对百草枯急性中毒大鼠早期肺间质纤维化的影响

摘要

目的：观察大鼠一次性灌胃百草枯染毒后，蒙脱石联合甘露醇对百草枯中毒大鼠肺间质纤维化的干预作用。方法雄性SD大鼠40只，按体质量随机分为对照组（C组），百草枯组（P组），百草枯染毒1 h后治疗组（PT1组）和4 h后治疗组（PT2组）。P组、PT1组、PT2组给予一次性灌胃染毒百草枯50 mg/kg ，于染毒后1、4 h分别给予PT1组、PT2组大鼠以7 g/kg蒙脱石甘露醇溶液灌胃治疗，C组及P组用生理盐水代替。观察大鼠一般情况变化。灌胃前后对大鼠进行跑步机训练，比较各组大鼠灌胃前，灌胃后5、10、15 d跑步机上掉落次数。于灌胃后第15天麻醉处死大鼠，收集肺组织，碱水解法测定肺组织羟脯氨酸含量， HE染色观察大鼠肺组织病理变化。结果 P组、PT1组和PT2组大鼠灌胃染毒后一般状况差，较对照组大鼠进食量明显降低（P＜0．01）。各组大鼠体质量增长、灌胃前在跑步机上掉落次数差异无统计学意义（P＞0．05）；灌胃后15 d ，P组大鼠跑步机上掉落次数较其余各组明显增加（P＜0．05）。各组大鼠肺组织羟脯氨酸含量总体差异显著（P＜0．01），其中，染毒各组较对照组肺组织羟脯氨酸含量明显增加（P＜0．05），P组较PT1、PT2组含量也明显增加（P＜0．05）。染毒各组大鼠肺组织切片呈现不同程度的肺纤维化改变，其中P组纤维化最为严重。结论百草枯急性中毒早期应用蒙脱石甘露醇溶液可减轻百草枯急性中毒大鼠肺间质纤维化程度。%Objective To observe the intervene effects of montmorillonite powder and mannitol on rat with acute paraquat poi-soning by intragastric administration .Methods 40 male SD rats were divided into four groups randomly ,control group(C) ,pa-raquat group(P) ,PT1 groups were treated with montmorillonite powder and mannitol gavage after paraquat exposure one hour ,PT2 group was treated after paraquat exposure four hours .P ,PT1 ,and PT2 groups were exposed to paraquat 50 mg/kg .one and four hour later ,PT1 and PT2 group rats were given 7 g/kg montmorillonite-mannitol solution respectively ,C group and P group with sa-line instead .then to observe general state in rats .Give rats run training before and after intragastric administration ,comparison the fall times from the treadmill among groups before and 5 ,10 ,15 days after administration ,rats were killed in 15 th days exposed pa-raquat ,collect their lung tissue ,hydroxyproline content were measured in lung tissue of rat by alkaline hydrolysis ,observe the path-ological changes by HE staining in rat lungs .Results the general condition were poor in P ,PT1 and PT2 group rats .And those mean food intakes were significantly lower than the control group (P<0 .01) .there are no significant of the weight gain and the fall times from the treadmill before and 5 th ,10 th after administration among four groups(P>0 .05) ,but P group′s fall times increased significantly than the others group′s at 15 th day after exposed(P< 0 .05) .the all group rat lung tissue hydroxyproline overall difference significant(P<0 .01) ,from the two-two compared results ,three paraquat exposed groups higher than control group ,and P group also higher than PT1 and PT2 group(P<0 .05) .From the lung tissue slices ,P ,PT1 and PT2 group showed significant pul-monary fibrosis than C group ,and the P group′s was the most severe .Conclusion Pulmonary interstitial fibrosis may be alleviated by early use of montmorillonite powder and mannitol on paraquat poisoning rats .