目的：探讨骨髓间充质干细胞（MSCs）对大鼠佐剂性关节炎（AA）的治疗、干预作用。方法收集培养扩增SD大鼠骨髓MSCs。SD大鼠注射弗氏完全佐剂造模之后行MSCs治疗及干预。造模后定期测量大鼠体质量、踝关节周长和后足爪体积。治疗后第21天，行四肢关节X线片及病理检查。结果模型大鼠经M SCs治疗后关节肿胀减轻，X线片示骨质破坏明显修复。病理HE染色见关节滑膜病变减轻。干预组经MSCs治疗，原发性炎症和继发病变均不明显，关节X线片和病理检查均未见明显异常，与模型组大鼠相比，体质量、足爪体积以及踝关节周长差异有统计学意义（P＜0．05）。结论 MSCs治疗可有效减轻并逆转弗氏完全佐剂诱导的大鼠AA的进展。%Objective To explore the mechanisms of action about how marrow mesenchymal stem cells (MSCs) treat and inter-vene the adjuvant arthritis (AA) of rats .Methods MSCs of SD rats which had been cultured and cloned would be collected .Fre-und′s complete adjuvant was administered in rats .The rats from the model group were given MSCs treatment and intervention .Dur-ing the treatment period ,those rats′ weights ,ankle circumferences and hind paw volumes were measured .On the 21st day of the treatment ,limb-joints X-ray examination and pathological examination were taken .Results After using MSCs to treat adjuvant ar-thritis of rats ,the joint swelling of the MSCs group were relieved ,and the limb-joints X-ray showed the destructive bones had ap-parent restoration .Pathological microscopic examination of HE staining ankle joint showed joint synovial lesions significantly reduced . In the MSCs intervention group ,rats′primary inflammation and secondary lesions were not obvious ,meanwhile ,joint X-ray and pathological examination showed no obvious abnormalities .Compared with the model group ,the differences of rats′weight ,paw volume and ankle circ-umference had statistically significant (P<0 .05) .Conclusion MSCs could relieve and reverse the progression of AA .
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