水飞蓟素对内皮祖细胞增殖、迁移功能的影响研究

摘要

目的：通过体外培养、鉴定内皮祖细胞（EPCs），进一步研究水飞蓟素对 EPCs活性和功能的影响，为进一步优化EPCs治疗缺血性疾病提供重要的理论依据。方法通过密度梯度离心法分离、培养、鉴定得到EPCs；在EPCs中加入不同浓度（0、25、50、100μg/m L ）水飞蓟素干预24 h后，检测细胞增殖、迁移和凋亡水平。结果与对照组比较，水飞蓟素干预24 h后，50～100μg/mL的水飞蓟素可明显增加EPCs的增殖和迁移能力（n＝6，P＜0．05），25～100μg/mL的水飞蓟素可明显抑制EPCs的凋亡（n＝6，P＜0．05），并呈浓度依赖性。结论水飞蓟素可呈浓度依赖性增强EPCs的活性和功能。%Objective Through culturing and identifying endothetial progenitor cells (EPCs) in vitro ,to study the impact of sily-marin on EPCs proliferation ,migration and apoptosis to proride the important theoretical basis for further optimizing the therapeutic effect of EPCs in treating ischemic diseases .Methods Peripheral blood was collected from human volunteer .Mononuclear cells (MNCs)were separated by density centrifugation and were induced to differentiate into EPCs in vitro .After the silymarin interven-tion with different concentrations(0 ,25 ,50 ,100 μg/mL)(50-100 μg/mL) for 24 h and the proliferation ,migration and apoptosis levels of EPCs were detected .Results Compared with the control group ,silymarin on EPCs could significantly enhance the prolifer-ation and migration ability of EPCs(n=6 ,P<0 .05) ,silymarin with the concentration of 25-100 μg/mL could significantly inhibit the apoptosis of EPCs in a concentration dependent manner (n=6 ,P<0 .05) .Conclusion Silymarin can enhance the activily and function EPCs in a concentration dependent manner .