目的:探讨蛇毒抗菌肽OH-CATH和壳聚糖(几丁糖)作为人工植入物涂层对大肠埃希菌的抑菌效果。方法预处理导尿管和涤纶补片(对照组、几丁糖组、头孢组和抗菌肽组)后进行体外抑菌试验,测定各组1d及将其置入血浆中7d后对大肠埃希菌标准株(E .coli ATCC 25922)和耐头孢菌素大肠埃希菌临床耐药株的抑菌活性值U。结果在E .coli ATCC 25922中,头孢组和抗菌肽组在1、7 d均有很强的抑菌效果,头孢组的抑菌活性均强于抗菌肽组( P<0.05);在E .co li耐药株中,头孢组无抑菌活性,而抗菌肽组在1、7 d有显著抑菌活性(P<0.01)。结论蛇毒抗菌肽OH-CATH对E .coli ATCC 25922及耐药株均有显著的抑菌效果,并肯定了它应用于人工植入物涂层的价值。%Objective To explore the bacteriostatic efficacy of snake venom antibacterial peptide OH-CATH and chitosan as ar-tificial implant coating on escherichia coli (E .coli) .Methods The catheters and dacron patches (blank group ,chitosan group ,cef-operazone group and antibacterial peptide group) were performed the pretreatment .Then the in vitro bacteriostatic test was conduc-ted to evaluate the antibacterial activity U value on E .coli ATCC 25922 and cephalosporin-resistant E .coli on 1 d and on 7 d after putting it into plasma .Results The cefoperazone group and the antibiotic peptide group had the very powerful antibacterial activity on E .coli ATCC 25922 on 1 ,7 d ,but the antibacterial activity of the cefoperazone group was stronger than that of the antibiotic peptide group(P<0 .05);the cefoperazone group had no antibacterial activity on cephalosporin-resistant E .coli ,but the antibiotic peptide group had significantly antibacterial activity on 1 ,7 d(P<0 .01) .Conclusion Snake venom antibacterial peptide OH-CATH has very obvious antibacterial effect on the standard strain and drug-resistant strain of E .coli and its value applied in artificial im-plant coating is affirmed .
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