唾液及血浆中微小RNA-21水平应用于早期食管癌的诊断价值

摘要

目的：研究唾液及血浆中的微小RNA‐21（miRNA‐21）水平应用于早期食管癌的诊断价值。方法选取2011年2月至2014年2月河南省南阳市中心医院收治的112例早期食管癌患者作为观察组，同期来该院进行健康查体的100例患者作为对照组，比较两组查体者miRNA‐21的表达水平，比较唾液和血浆miRNA‐21的诊断价值；研究miRNA‐21水平与肿瘤分期、病理类型和分化的相关性。结果观察组唾液miRNA‐21水平为（6．08±2．22），对照组为（0．64±0．09），差异有统计学意义（P＜0．05）；观察组的血浆miRNA‐21表达水平为（20．91±10．59），对照组为（1．69±0．17），差异有统计学意义（P＜0．05）。唾液中的miRNA‐21水平ROC曲线下面积（AUC）为0．8665，灵敏度为88．24％，特异度为69．97％；血浆中的miRNA‐21 AUC为0．8820，灵敏度为90．20％，特异度为70．69％，二者诊断价值差异无统计学意义（P＞0．05）。唾液与血浆中的miRNA‐21水平与早期食管癌的分期和病理类型无明显的相关性（P＞0．05），与分化程度相关性较强（P＜0．05）。结论唾液及血浆中的miRNA‐21水平应用于早期食管癌具有较高的诊断价值，唾液可能代替血浆应用于早期食管癌的诊断。%Objective To study the diagnostic values of salivary and plasma microRNA‐21 for e early esophageal cancer . Methods Totally 112 patients with early esophageal cancer in Nanyang Municipal Central Hospital from February 2011 to Febru‐ary 2014 were selected as the observation group and contemporaneous 100 healthy people of physical examination were selected as the contract group .The salivary and plasma microRNA‐21 expression levels were compared between the two groups ;the diagnostic values of salivary versus plasma microRNA‐21 for the early esophageal cancer were also compared;the correlation between the miR‐NA‐21 level with the stage ,pathogenic type and differentiation of early esophageal cancer .Results The salivary microRNA‐21 level (6 .08 ± 2 .22) in the observation group and (0 .64 ± 0 .09) in the control group ,the difference had statistical significance (P<0 .05);the plasma microRNA‐21 level in the observation group was (20 .91 ± 10 .59) and (1 .69 ± 0 .17) in the constract group ,the difference had statistical significance (P<0 .05) .The area underROC curve(AUC) of salivary microRNA‐21 level was 0 .866 5 ,the sensitivity was 88 .24% ,the specificity was 69 .97% ;while AUC of plasma microRNA‐21 level was 0 .882 0 ,the sensitivity was 90 .20% ,the specificity was 70 .69% ,the differences in the diagnostic value had no statistical significance between them(P>0 .05) . The salivary and plasma microRNA‐21 levels had no obvious correlation with the stage and pathogenic type of early esophageal cancer(P>0 .05) ,while had strong correlation with the differentiation degree(P<0 .05) .Conclusion Salivary and plasma microR‐NA‐21 has higher diagnostic value in for early esophageal cancer ,and saliva may supplant plasma in the diagnosis of early esophage‐al cancer .